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 Post subject: Media and hormones for Darlingtonia seedlings
PostPosted: 16 Oct 2009, 20:33 
Joined: 12 Oct 2009, 16:36
Posts: 44
Location: Ashland, OR, USA
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Anyone have tips for growing Darlingtonia in vitro? Right now I have some growing on 1/2 MS with vitamins and they seem pretty happy. They are only about 2 months old, so they're still quite small. What media have others found to work best for them? Do you use any kind of hormones? On my forum, Jens (nepaholic) post a photo of a Darlingtonia seedling that he put on hormones (TDZ - Thidiazuron and BAP):
Image
Image

Anyone ever used hormones on Darlingtonia seedlings? And if so, what was your preferred mix?

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 Post subject: Re: Media and hormones for Darlingtonia seedlings
PostPosted: 16 Oct 2009, 21:01 
Joined: 02 Oct 2009, 19:16
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Darlingtonia multiply very well without hormones on 1/2MS in my conditions.


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 Post subject: Re: Media and hormones for Darlingtonia seedlings
PostPosted: 16 Oct 2009, 21:04 
Joined: 12 Oct 2009, 16:36
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Location: Ashland, OR, USA
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Thanks Milos. Mine seem to be progressing quite quickly on 1/2 MS as well. I just thought I'd see if anyone else had better techniques for rushing their growing or multiplication.

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 Post subject: Re: Media and hormones for Darlingtonia seedlings
PostPosted: 18 Oct 2009, 21:04 
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Before few years I tested the response to cytokinin.
1/3MS medium supplemented with cytokinin 2.3 µM and auxin 0.5 µM
Cytokinin
KINETIN [ 6-furfurylaminopurine ]
trans-ZEATIN [6-((E)-4-hydroxy-3-methylbut-2-enylamino)purine]
N6 BENZYLADENINE [6 benzylaminopurine]
MoBO meta-methoxy benzylaminopurin-ribosid
Auxin
IBA - [4-(3-indolyl)butyric acid]


KINETIN Image BAPImage

MoBO Image trans-ZEATIN Image

Most effective was combination trans ZEATIN + IBA

Michal

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 Post subject: Re: Media and hormones for Darlingtonia seedlings
PostPosted: 18 Oct 2009, 21:36 
Joined: 12 Oct 2009, 16:36
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Location: Ashland, OR, USA
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Wow Michal! Thanks for the great information! I really appreciate it.

I don't have any ZEATIN to work with and it's a bit out of my price range. I do have kinetin and IBA and it appears from your photos that you got good results with it as well.

I'll give it a try!

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 Post subject: thank U very much
PostPosted: 06 Jan 2011, 14:16 
Joined: 03 Jan 2011, 08:32
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Great share,thank U very much!


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