FORUM IN VITRO
https://www.labflytrap.com/forum/

Preferred Cephalotus media and hormones
https://www.labflytrap.com/forum/viewtopic.php?f=17&t=52
Page 1 of 1

Author:  flytrapcare.com [ 16 Oct 2009, 20:40 ]
Post subject:  Preferred Cephalotus media and hormones

I've never had any Cephalotus in vitro yet, but I will be receiving some soon. I've read that 20% MS is good for cephs, but that they grow slowly. Has anyone experimented with different hormones and media? If so, what was the best performing combination that you were able to find?

Author:  dUKe [ 20 Oct 2009, 05:16 ]
Post subject:  Re: Preferred Cephalotus media and hormones

im used 2/3 Knudson + MS vitamin, 2 ml/L ba, 200 mg activated charcoal, 5.4-5.5 pH, 6.8 G/L agar. Free-hormone or 0.2 naa for pretransplant Well.

Author:  Milos Sula [ 20 Oct 2009, 06:49 ]
Post subject:  Re: Preferred Cephalotus media and hormones

Hi dUKe, please specfy what Knudson you have used.
Knudson C ( original formulation from 1946 ) or any modification of Knudson C ( Morel ) ? There is a big difference between them.

Author:  flytrapcare.com [ 20 Oct 2009, 15:56 ]
Post subject:  Re: Preferred Cephalotus media and hormones

Thanks for the information dUKe!

One question: What is 2 ml/L ba? Is that 6-BAP?

Author:  dUKe [ 21 Oct 2009, 05:10 ]
Post subject:  Re: Preferred Cephalotus media and hormones

Milos Sula wrote:
Hi dUKe, please specfy what Knudson you have used.
Knudson C ( original formulation from 1946 ) or any modification of Knudson C ( Morel ) ? There is a big difference between them.


im used Knudson, original formula 1946

flytrapcare.com wrote:
Thanks for the information dUKe!

One question: What is 2 ml/L ba? Is that 6-BAP?


ba=N6-benzyladenine

Author:  drosophyllum [ 06 Nov 2010, 20:09 ]
Post subject:  Re: Preferred Cephalotus media and hormones

dUKe wrote:
im used 2/3 Knudson + MS vitamin, 2 ml/L ba, 200 mg activated charcoal, 5.4-5.5 pH, 6.8 G/L agar. Free-hormone or 0.2 naa for pretransplant Well.


Why do you work with activated charcoal? Is it essential or only recommended?

Thanks

Page 1 of 1 All times are UTC + 1 hour
Powered by phpBB © 2000, 2002, 2005, 2007 phpBB Group
http://www.phpbb.com/