FORUM IN VITRO

Hi,
I just received a few seeds of Drosophyllum and I want to sow it in TC. Does anybody know what is the best techniqe to sterilize these seeds, and what medium should I use to sow them ?

Many thanks
Mateusz

I sow it last week and they began to germinate 7-8 day after sowing. I use MS macro/2.

I sanded seeds with 2 emery-paper prior to sterilization with bleach.
This work fine, seeds look swelled.

Do you think 50%MS will be ok ? How long did you sterilized your seeds in bleach solution ?

There is a publication about multiplication in vitro of drosophyllum. They conclude that full MS strengh was the most efficient medium. try with full strengh.

I sterilized seeds for about 3 min.

Thanks. I received this article today, so in one week I'll start new culture. I'll write if I have a success.