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Milos Sula
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Post subject: Dionaea muscipula  Posted: 27 Oct 2009, 19:53 |
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Joined: 02 Oct 2009, 19:16 Posts: 61
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robust red clone A - Mirek Srba - 1/2MS  B52 - 1/2MS 
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PHaze
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Post subject: Re: Dionaea muscipula Posted: 27 Oct 2009, 22:32 |
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Joined: 21 Oct 2009, 23:18 Posts: 12
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Did you use any growth regulators to get them to multiply like that?
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Milos Sula
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Post subject: Re: Dionaea muscipula Posted: 27 Oct 2009, 22:40 |
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Joined: 02 Oct 2009, 19:16 Posts: 61
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No,
this is pure 1/2MS medium.
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goldslinger
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Post subject: Re: Dionaea muscipula Posted: 04 Nov 2009, 02:18 |
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Joined: 26 Oct 2009, 02:43 Posts: 5
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Very, Very, Nice !
Thanks for sharing!
Were they from seed or leaf cutting?
How many times have You re-plated or are they in the original media?
How old is that culture? (I thik I read a date of July, 21).
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Milos Sula
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Post subject: Re: Dionaea muscipula Posted: 04 Nov 2009, 13:02 |
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Joined: 02 Oct 2009, 19:16 Posts: 61
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Both cultures were replated 2 times - each 3 months.
The first one has been initiated form flower stalk ( ~ 6 months old ), the second i have got from my friend.
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goldslinger
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Post subject: Re: Dionaea muscipula Posted: 24 Nov 2009, 03:55 |
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Joined: 26 Oct 2009, 02:43 Posts: 5
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That's great!
Did You bring the Vitamins back up to 100% or left them at 50% as well as the MS?
Thanks!
Gary
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Milos Sula
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Post subject: Re: Dionaea muscipula Posted: 25 Nov 2009, 12:05 |
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Joined: 02 Oct 2009, 19:16 Posts: 61
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Full MS vitamins, 1/2MS macro, 1/2MS micro
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cplover
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Post subject: Re: Dionaea muscipula Posted: 27 Nov 2009, 02:39 |
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Joined: 24 Nov 2009, 05:49 Posts: 7
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Hi Milos, all member in forum
I really interest CP tissue culture, specially like flytrap and nepenthes TC.
This forum very helpful to someone like me.
However, I just study CT 2 month, have no time and condition for do it at home. But I planning to start make a small lab for test at home. In future I will need more help.
I hope this forum will more and more development!
Best ragards!
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goldslinger
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Post subject: Re: Dionaea muscipula Posted: 29 Nov 2009, 06:39 |
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Joined: 26 Oct 2009, 02:43 Posts: 5
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Milos Sula wrote: Full MS vitamins, 1/2MS macro, 1/2MS micro That's good to know, Thanks! Oh, and welcome to the forums, CP Lover! Gary
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cplover
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Post subject: Re: Dionaea muscipula Posted: 02 Dec 2009, 04:18 |
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Joined: 24 Nov 2009, 05:49 Posts: 7
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Hi all!
I planning to use Flytrap leaf for culture, what media can I use(media for scar tissue)? And how long can I replated? This is first time I do it, so I have no experience of this.
If possible, please give me some suggests of sterilization.
Many thanks!
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