FORUM IN VITRO

Drosera anglica. Young plants growing from tissue of 2 leaf explants. Disinfection: 70% EtOH dip, 2mins 0,5% NaOCl and 2x water rinse. Medium used was 1/3MS (home-made) and vitamins (no B8), sucrose 30g/l, agar 6g/l, no hormones.
Adventitious buds formed on leaves in 21 days:



The buds evolved into shoots and roots (2 weeks after sprouting):



one month


two months old plants (although there was light contamination of yiest, the plants grow on well)

Hi there! I deflasked the d. anglica jar after 2 months. I left some plants for subculture and I potted the rest up. They developed only shoots and no roots on the hormoneless media. Therefore I am curious how many plants will develop roots and catch on peat media. The shoot formation ratio was 14 plants/explant. I attach a pic of freshly deflasked plants:

I post a pic of d. anglica culture coming out of dormancy and proliferating vividly. I used to use hormones for d. anglica, but it seems that medium without hormones works much better for me.

I subcultures the plants and put them on fresh media (many were de-flasked), they grow better than outside of the glass:-) They broke dormancy just recently.

Hey Dusan,
I've been searching for sundew-specific leaf disinfection techniques and finally stumbled across this page.

I am finally getting around to trying leaf tissue culture on D. aliciae and D. capensis for practice and was wondering how long you left the leaves in the bleach solution?
Also, if I were to skip the EtOH rinse, and use a 400 mL batch of 10% bleach (approx .5% NaOCl) and 2 drops of Tween20, how long would you expect it should take to disinfect a batch of 10 leaves?
I ended up shaking/stirring them in bleach for 10 min. and doing 5-6 rinses with DI H20.
Thanks,
Aaron