FORUM IN VITRO

Hello

I have some D.regia in in-vitro culture. I tried propagation from leaf explants on medium 40%MS with 0.015mg kinetin 0.001 IBA/l, but it falied- i didn't get any plantlets. I have to use more kinetin, yes? Now i'm trying with whole plants on 0,5mg kinetin/l,.

If anybody know hormones concentrartion for micropropagation of D.regia from leaves i will be very thankful

Hi.
D. regia responds on 2mg/l 2iP + 0,1 mg/l 24D.
Medium 1/3 MS with addition 100mg/l KCl.



D. pauciflora the same way too.

Yeah Great that it is possible. I have some 4-CPA, not 2,4-D, i also don't have 2-iP. But 2-ip have low- activity and adenine-based structure, i will try 0,5 mg 6-BAP/l + 0,1mg 4-CPA. Mayby it will work if not i have to order 2iP

Does an adenine-based structure cause problems?

No But BAP is also based on adenine ring,



And structure of iP is very similar, becouse there is isopenthyl group in the place of benzyl group, so their mechanism of action is similar- i hope that BA will work too.

Guys, do you have the instruction for root cuttings? Size of the root, sterilization?

Thanks!

hb

4.02 i planted some D.regia leaves on medium with BAP and 4-CPA. Todat i saw some strike! Leaves are completly red in 90%, and doesn't look well, but in 1/10 i can see ~10 small green plantlets. Yeah

Sativ, have you some news from your experience?

Since 1 month, i have 2 leaves of regia on MS/3 + 1mg/l BAP and they are green and look good. But also no signe of bud...

I'll wait and we'll see