FORUM IN VITRO

Cultivation of plants in different medias.
Become a part of a project.
I will send in-vitro plant materials for experimentation.
Necessary condition:
Publication of results of the experiment in the forum, including photographs.

Medium

All medias- full MS vitamins, 30g surcose/l, 6g agar/l pH 5,6
step 1
20% MS with full MS vitamins, 30g/L sugar, pH 5.6, 6g/l agar
30% MS with full MS vitamins, 30g/L sugar, pH 5.6, 6g/lagar
40% MS with full MS vitamins, 30g/L sugar, pH 5.6, 6g/l agar
step 2
Best concentration of hormones for multiplification
step 3
Best concentration of homones for rooting

Sending you a PM!

I received the tissue today and I was able to put the tissue onto the following media:
1 culture - 50% Knudson C (original forumla) + full MS vitamins, 30g/L sugar
1 culture - 50% WPM + 50% vitamins, 30g/L sugar
2 cultures - 10% MS + full MS vitamins, 30g/L sugar
2 cultures - 20% + full MS vitamins, 30g/L sugar
3 cultures - 30% + full MS vitamins, 30g/L sugar
4 cultures - 40% + full MS vitamins, 30g/L sugar
3 cultures - 50% + full MS vitamins, 30g/L sugar, 1ml/L PPM, 1.5g/L activated charcoal
pH = 5.6-5.7 for all cultures

I'll post again when there's something to report!

A long overdue update.

The H. minor tissue seemed to grow well on nearly everything I put it on. It did best on 30% to 50% MS and 50% WPM. It didn't do very well on 10% MS, 20% MS and 50% Knudson C, growing slowly and with poor color.

Interestingly, the tissue didn't grow roots on any of the MS media or the Knudson C media, but it did grow roots on the WPM. Recently, I've put quite a bit of the tissue onto 10% WPM, 25% WPM and 40% WPM to verify that grows well and grows roots and I have confirmed this. Here are a few photos of the cultures before their recent replate.

Hi flytrapcare,
please have you tried 50% Knudson C with MS micronutrients or original Knudson C micronutrients ?


Milos

Hello Milos,

I'm using this formula:
http://www.phytotechlab.com/TechInfo/K400-Info.pdf
and adding MS micronutrients:
http://www.phytotechlab.com/TechInfo/M533-Info.pdf

Yes, such results i have expected.

WPM is good rooting medium probably due higher potassium content and low salts content

Knudson C, diluted MS ( 10,20% ) are very poor mediums. Knudson C is poor in micronutrients, diluted MS in both macro and micronutrients.It will be better to use Knudson C with for example 1/2MS micro and full MS vitamins and diluted MS ( 10%,20% ) also with 1/2 MS micro and full MS vitamins.

BTW. The Knudson C media you are using is not original formula, this is later Morel modification.And you have written link to the MS vitamins not to the MS micronutrients.

Right you are!

I'm going to show my lack of knowledge here. I thought vitamins were the same thing as micronutrients
In any case, I realize now that they are different. The MS mixture I'm using has macro and micronutrients and vitamins:
http://www.phytotechlab.com/MSDS/M519msds.pdf
But when I cut it to 50%, I bring the vitamins back up to 100% with this:
http://www.phytotechlab.com/TechInfo/M533-Info.pdf

I don't even know where to purchase MS micronutrients.

For example here are micronutrients:
http://www.phytotechlab.com/detail.aspx?ID=456

For testing/optimalization of media is better to buy macronutrients http://www.phytotechlab.com/detail.aspx?ID=441 ,micronutrient and vitamins separately.
It is good idea to test different media macronutrients first with the same vitamins and micronutrients.
For example:
Knudson C macro + 1/2MS micro + MS vitamins
20%MS macro + 1/2MS micro + MS vitamins
WPM macro + 1/2MS micro + MS vitamins

etc...
Then you will select the best medium ( here i have selected 20%MS ) for your plant and you can continue testing for different micronutrients:
20%MS macro + 1/2MS micro + MS vitamins
20%MS macro + MS micro + MS vitamins
20%MS macro + 1/2WPM micro + MS vitamins
20%MS macro + WPM micro + MS vitamins

after this you will select the best micronutrients for your plant and the testing continues with different vitamins...