FORUM IN VITRO

Micropropagation of carnivorous plants


It is currently 28 Mar 2024, 22:57

All times are UTC + 1 hour




Post new topic Reply to topic  [ 16 posts ]  Go to page Previous  1, 2


 Post subject: Re: Introduction of nepenthes to in vitro from explants
PostPosted: 24 Apr 2010, 11:16 
Joined: 02 Oct 2009, 19:16
Posts: 61
Offline
What do you mean with failed ?

Contamination or the explants died ?


Top
 Profile  
 

 Post subject: Re: Introduction of nepenthes to in vitro from explants
PostPosted: 24 Apr 2010, 11:46 
Joined: 24 Nov 2009, 05:49
Posts: 7
Offline
Not contamination, but the explant dead (green color change to black color). Maybe my method to strong for nepenthes?

I want to try TC with nep leaf, do you think is it possible?


Top
 Profile  
 

 Post subject: Re: Introduction of nepenthes to in vitro from explants
PostPosted: 25 Apr 2010, 21:49 
Joined: 12 Oct 2009, 19:24
Posts: 42
Offline
As i know for now- it is not possible, there were some info about geting cellus from tendrils but i didn't veryfied it yet- it also needs many experiments to do...

What exacly was the explant? I had fail with this method- contamination...


Top
 Profile  
 

 Post subject: Re: Introduction of nepenthes to in vitro from explants
PostPosted: 26 Apr 2010, 02:58 
Joined: 24 Nov 2009, 05:49
Posts: 7
Offline
my explant are N. trichocarpa and N. mirabilis. all dead without contamination.


Top
 Profile  
 

 Post subject: Re: Introduction of nepenthes to in vitro from explants
PostPosted: 07 Jul 2010, 19:35 
Joined: 07 Jul 2010, 19:16
Posts: 1
Offline
Is anyone aware of just how quickly Nepenthes becomes host to that endosymbiotic fungi and whether the use of a conventionally germinated seedling plant -- months old -- would improve results for in vitro use as an explant than, say, an older, more established one?


Top
 Profile  
 

 Post subject: Re: Introduction of nepenthes to in vitro from explants
PostPosted: 08 Aug 2013, 09:45 
Joined: 28 Jul 2011, 10:02
Posts: 10
Offline
Chloramphenicol is toxic but tetracykline is harmful only.

_________________
wholesale Ed Hardy


Top
 Profile  
 

Display posts from previous:  Sort by  

Post new topic Reply to topic  [ 16 posts ]  Go to page Previous  1, 2

All times are UTC + 1 hour


Who is online

Users browsing this forum: No registered users and 1 guest


You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot post attachments in this forum

Search for:
Jump to:  
cron
Powered by phpBB © 2000, 2002, 2005, 2007 phpBB Group