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| Introduction of nepenthes to in vitro from explants https://www.labflytrap.com/forum/viewtopic.php?f=7&t=55 |
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| Author: | Milos Sula [ 24 Apr 2010, 11:16 ] |
| Post subject: | Re: Introduction of nepenthes to in vitro from explants |
What do you mean with failed ? Contamination or the explants died ? |
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| Author: | cplover [ 24 Apr 2010, 11:46 ] |
| Post subject: | Re: Introduction of nepenthes to in vitro from explants |
Not contamination, but the explant dead (green color change to black color). Maybe my method to strong for nepenthes? I want to try TC with nep leaf, do you think is it possible? |
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| Author: | sativ [ 25 Apr 2010, 21:49 ] |
| Post subject: | Re: Introduction of nepenthes to in vitro from explants |
As i know for now- it is not possible, there were some info about geting cellus from tendrils but i didn't veryfied it yet- it also needs many experiments to do... What exacly was the explant? I had fail with this method- contamination... |
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| Author: | cplover [ 26 Apr 2010, 02:58 ] |
| Post subject: | Re: Introduction of nepenthes to in vitro from explants |
my explant are N. trichocarpa and N. mirabilis. all dead without contamination. |
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| Author: | bigbella [ 07 Jul 2010, 19:35 ] |
| Post subject: | Re: Introduction of nepenthes to in vitro from explants |
Is anyone aware of just how quickly Nepenthes becomes host to that endosymbiotic fungi and whether the use of a conventionally germinated seedling plant -- months old -- would improve results for in vitro use as an explant than, say, an older, more established one? |
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| Author: | Vincentcnbres [ 08 Aug 2013, 09:45 ] |
| Post subject: | Re: Introduction of nepenthes to in vitro from explants |
Chloramphenicol is toxic but tetracykline is harmful only. |
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