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Micropropagation of carnivorous plants


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 Post subject: Nepenthes and TDZ
PostPosted: 13 Apr 2010, 12:20 
Joined: 02 Oct 2009, 19:16
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Hi , i have tried 0.1mg /L TDZ with nepenthes.
But this does not look optimal ( too much callus ).
It is better to add auxin, or do i need to lower the concentrationm of TDZ ?

N.tomoriana
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 Post subject: Re: Nepenthes and TDZ
PostPosted: 13 Apr 2010, 14:19 
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Joined: 30 Sep 2009, 23:26
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Hi,
What is concentration of macro and microelements.

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 Post subject: Re: Nepenthes and TDZ
PostPosted: 13 Apr 2010, 17:11 
Joined: 02 Oct 2009, 19:16
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In this case i have used weak medium created by Ronald de Fossard for nepenthes cultivation.
Code:
type   component   mg/l   g/mol   mmol

macro   KNO3      202   101,1   2,00E+00
macro   NH4NO3      160   80,04   2,00E+00
macro   NaH2PO4.H2O   156   137,99   1,13E+00
macro   MgSO4.7H2O   74   246,48   3,00E-01
macro   CaCl2.2H2O   44   147,02   2,99E-01

micro   FeNaEDTA.3H2O   42   421,1   9,97E-02
micro   MnSO4.4H2O   22,3   223,06   1,00E-01
micro   ZnSO4.7H2O   11,5   287,54   4,00E-02
micro   H3BO3      9,27   61,83   1,50E-01
micro   KI      0,83   166   5,00E-03
micro   Na2MoO4.2H2O   0,242   241,95   1,00E-03
micro   CuSO4.5H2O   0,025   249,68   1,00E-04
micro   CoCl2.6H2O   0,0238   237,98   1,00E-04


+ MS vitamins + 25g/l sugar


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 Post subject: Re: Nepenthes and TDZ
PostPosted: 14 Apr 2010, 20:32 
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Joined: 30 Sep 2009, 23:26
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Hi.
TDZ is problem and isn't acceptable use for CPs.
TDZ is powerful herbicide developed on defoliation cotton - plant before harvest.

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 Post subject: Re: Nepenthes and TDZ
PostPosted: 18 Apr 2010, 20:21 
Joined: 12 Oct 2009, 19:24
Posts: 42
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I find TDZ very good for sarracenia multiplification, but i didn't tried it with nepenthes yet- and i don't think that i will try ;)


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